1QCU

CRYSTAL STRUCTURE OF AN 18 BASE PAIR COPY CONTROL RELATED RNA DUPLEX

1QCU の概要

• エントリーDOI: 10.2210/pdb1qcu/pdb
• 関連するPDBエントリー: 1QC0
• 分子名称: 5'-R(P*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*G)-3', 5'-R(P*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*C)-3', AMMONIUM ION, ... (4 entities in total)
• 機能のキーワード: a-rna structure, ribonucleic acid, rna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 14200.85

構造登録者

Klosterman, P.S.,Shah, S.A.,Steitz, T.A. (登録日: 1999-05-14, 公開日: 1999-11-11, 最終更新日: 2024-04-03)

主引用文献

Klosterman, P.S.,Shah, S.A.,Steitz, T.A.
Crystal structures of two plasmid copy control related RNA duplexes: An 18 base pair duplex at 1.20 A resolution and a 19 base pair duplex at 1.55 A resolution.
Biochemistry, 38:14784-14792, 1999

PubMed Abstract: The structures of two RNA duplexes, whose sequences correspond to portions of the ColE1 plasmid copy control RNA I and RNA II, have been determined. Crystals containing the 18mers 5'-CA CCGUUGGUAGCGGUGC-3' and 5'-CACCGCUACCAACGGUGC-3' diffract to 1.20 A resolution while those containing the 19mers 5'-GCACCGUUGGUAGCGGUGC-3' and 5'-GCACCGCUACCAACGGUGC-3' diffract to 1.55 A resolution. Both duplexes are standard A form, with Watson-Crick base pairing throughout. Use of anisotropic atomic displacement factors in refinement of the 1.20 A structure dramatically improved refinement statistics, resulting in a final R(free) of 15.0% and a crystallographic R-factor of 11.6%. Perhaps surprisingly, these crystals of the 18 base pair RNA exhibit a 36-fold static disorder, resulting in a structure with a single sugar-phosphate backbone conformation and an averaged base composition at each residue. Since the sugar-phosphate backbone structure is identical in the 36 different nucleotides that are superimposed, there can be no sequence-dependent variation in the structure. The average ribose pucker amplitude is 45.8 degrees for the 18 base pair structure and 46.4 degrees for the 19 base pair structure; these values are respectively 19% and 20% larger than the average pucker amplitude reported from nucleoside crystal structures. A standard RNA water structure, based on analysis of the hydration of these crystal structures and that of the TAR RNA stem [Ippolito, J. A., and Steitz, T. A. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 9819-9824], has been derived, which has allowed us to predict water positions in lower resolution RNA crystal structures. We report a new RNA packing motif, in which three pro-S(p) phosphate oxygens interact with an ammonium ion.

PubMed: 10555960
DOI: 10.1021/bi9912793

実験手法

X-RAY DIFFRACTION (1.2 Å)