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1QB2

CRYSTAL STRUCTURE OF THE CONSERVED SUBDOMAIN OF HUMAN PROTEIN SRP54M AT 2.1A RESOLUTION: EVIDENCE FOR THE MECHANISM OF SIGNAL PEPTIDE BINDING

1QB2 の概要
エントリーDOI10.2210/pdb1qb2/pdb
分子名称HUMAN SIGNAL RECOGNITION PARTICLE 54 KD PROTEIN (2 entities in total)
機能のキーワードalpha-helix, helix-turn-helix, signaling protein
由来する生物種Homo sapiens (human)
細胞内の位置Nucleus speckle : P61011
タンパク質・核酸の鎖数2
化学式量合計24946.88
構造登録者
Clemons Jr., W.M.,Gowda, K.,Black, S.D.,Zwieb, C.,Ramakrishnan, V. (登録日: 1999-04-29, 公開日: 1999-10-20, 最終更新日: 2024-02-14)
主引用文献Clemons Jr., W.M.,Gowda, K.,Black, S.D.,Zwieb, C.,Ramakrishnan, V.
Crystal structure of the conserved subdomain of human protein SRP54M at 2.1 A resolution: evidence for the mechanism of signal peptide binding.
J.Mol.Biol., 292:697-705, 1999
Cited by
PubMed Abstract: Protein SRP54 is an integral part of the mammalian signal recognition particle (SRP), a cytosolic ribonucleoprotein complex which associates with ribosomes and serves to recognize, bind, and transport proteins destined for the membrane or secretion. The methionine-rich M-domain of protein SRP54 (SRP54M) binds the SRP RNA and the signal peptide as the nascent protein emerges from the ribosome. A focal point of this critical cellular function is the detailed understanding of how different hydrophobic signal peptides are recognized efficiently and transported specifically, despite considerable variation in sequence. We have solved the crystal structure of a conserved functional subdomain of the human SRP54 protein (hSRP54m) at 2.1 A resolution showing a predominantly alpha helical protein with a large fraction of the structure available for binding. RNA binding is predicted to occur in the vicinity of helices 4 to 6. The N-terminal helix extends significantly from the core of the structure into a large but constricted hydrophobic groove of an adjacent molecule, thus revealing molecular details of possible interactions between alpha helical signal peptides and human SRP54.
PubMed: 10497032
DOI: 10.1006/jmbi.1999.3090
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.1 Å)
構造検証レポート
Validation report summary of 1qb2
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-09に公開中

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