1QAZ

CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION

1QAZ の概要

• エントリーDOI: 10.2210/pdb1qaz/pdb
• 分子名称: PROTEIN (ALGINATE LYASE A1-III), SULFATE ION (3 entities in total)
• 機能のキーワード: alginate lyase, crystals, lyase
• 由来する生物種: Sphingomonas sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39841.72

構造登録者

Yoon, H.-J. (登録日: 1999-04-08, 公開日: 1999-07-19, 最終更新日: 2024-11-06)

主引用文献

Yoon, H.J.,Mikami, B.,Hashimoto, W.,Murata, K.
Crystal structure of alginate lyase A1-III from Sphingomonas species A1 at 1.78 A resolution.
J.Mol.Biol., 290:505-514, 1999

PubMed Abstract: The three-dimensional structure of alginate lyase A1-III (ALYIII) from a Sphingomonas species A1 was determined by X-ray crystallography. The enzyme was crystallized by the hanging-drop vapour-diffusion method in the presence of 49% ammonium sulfate at 20 degrees C. The crystals are monoclinic and belong to the space group C2 with unit cell dimensions of a=49.18 A, b=93.08 A, c=82.10 A and beta=104.12 degrees. There was one molecule of alginate lyase in the asymmetric unit of the crystal. The diffraction data up to 1. 71 A were collected with Rsymof 5.0%. The crystal structure of ALYIII was solved by the multiple isomorphous replacement method and refined at 1.78 A resolution using X-PLOR with a final R -factor of 18.0% for 10.0 to 1.78 A resolution data. The refined model of ALYIII contained 351 amino acid residues, 299 water molecules and two sulfate ions. The three-dimensional structure of ALYIII was abundant in helices and had a deep tunnel-like cleft in a novel (alpha6/alpha5)-barrel structure, which was similar to the (alpha6/alpha6)-barrel found in glucoamylase and cellulase. This structure presented the possibility that alginate molecules might penetrate into the cleft to interact with the catalytic site of ALYIII.

PubMed: 10390348
DOI: 10.1006/jmbi.1999.2883

実験手法

X-RAY DIFFRACTION (1.78 Å)