1Q9H

3-Dimensional structure of native Cel7A from Talaromyces emersonii

1Q9H の概要

• エントリーDOI: 10.2210/pdb1q9h/pdb
• 分子名称: cellobiohydrolase I catalytic domain, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: cellobiohydrolase i catalytic domain, hydrolase
• 由来する生物種: Talaromyces emersonii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47495.28

構造登録者

Grassick, A.,Thompson, R.,Murray, P.G.,Collins, C.M.,Byrnes, L.,Tuohy, M.G.,Birrane, G.,Higgins, T.M. (登録日: 2003-08-25, 公開日: 2004-11-09, 最終更新日: 2024-10-30)

主引用文献

Grassick, A.,Murray, P.G.,Thompson, R.,Collins, C.M.,Byrnes, L.,Birrane, G.,Higgins, T.M.,Tuohy, M.G.
Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii
Eur.J.Biochem., 271:4495-4506, 2004

PubMed Abstract: The X-ray structure of native cellobiohydrolase IB (CBH IB) from the filamentous fungus Talaromyces emersonii, PDB 1Q9H, was solved to 2.4 A by molecular replacement. 1Q9H is a glycoprotein that consists of a large, single domain with dimensions of approximately 60 A x 40 A x 50 A and an overall beta-sandwich structure, the characteristic fold of Family 7 glycosyl hydrolases (GH7). It is the first structure of a native glycoprotein and cellulase from this thermophilic eukaryote. The long cellulose-binding tunnel seen in GH7 Cel7A from Trichoderma reesei is conserved in 1Q9H, as are the catalytic residues. As a result of deletions and other changes in loop regions, the binding and catalytic properties of T. emersonii 1Q9H are different. The gene (cel7) encoding CBH IB was isolated from T. emersonii and expressed heterologously with an N-terminal polyHis-tag, in Escherichia coli. The deduced amino acid sequence of cel7 is homologous to fungal cellobiohydrolases in GH7. The recombinant cellobiohydrolase was virtually inactive against methylumberiferyl-cellobioside and chloronitrophenyl-lactoside, but partial activity could be restored after refolding of the urea-denatured enzyme. Profiles of cel7 expression in T. emersonii, investigated by Northern blot analysis, revealed that expression is regulated at the transcriptional level. Putative regulatory element consensus sequences for cellulase transcription factors have been identified in the upstream region of the cel7 genomic sequence.

PubMed: 15560790
DOI: 10.1111/j.1432-1033.2004.04409.x

実験手法

X-RAY DIFFRACTION (2.35 Å)