1Q8F

Crystal Structure of the E.coli pyrimidine nucleoside hydrolase yeiK

1Q8F の概要

• エントリーDOI: 10.2210/pdb1q8f/pdb
• 関連するPDBエントリー: 1HP0 1MAS 2MAS
• 分子名称: Pyrimidine nucleoside hydrolase, CALCIUM ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: open alpha-beta structure, nh-fold, hydrolase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 136681.22

構造登録者

Giabbai, B.,Degano, M. (登録日: 2003-08-21, 公開日: 2004-05-11, 最終更新日: 2023-08-16)

主引用文献

Giabbai, B.,Degano, M.
Crystal structure to 1.7 a of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy.
STRUCTURE, 12:739-749, 2004

PubMed Abstract: Enzymes with nucleoside hydrolase (NH) activity are crucial for salvaging nucleic acid components in purine auxotrophic protozoan parasites, but are also present in prokaryotes and higher eukaryotes. Here we analyze the distribution of genes encoding for putative NH proteins and characterize the yeiK gene product from Escherichia coli as a pyrimidine-specific NH. The crystal structure of YeiK to 1.7 A defines the structural basis for its substrate specificity and identifies residues involved in the catalytic mechanism that differ from both nonspecific and purine-specific NHs. Large differences in the tetrameric quaternary structure compared to nonspecific protozoan NHs are brought forth by minor differences in the interacting surfaces. The first structural and functional characterization of a nonparasitic, pyrimidine nucleoside-specific NH suggests a possible role for these enzymes in the metabolism of tRNA nucleosides. The high catalytic efficiency of YeiK toward 5-fluorouridine could be exploited for suicide gene therapy in cancer treatment.

PubMed: 15130467
DOI: 10.1016/j.str.2004.03.018

実験手法

X-RAY DIFFRACTION (1.7 Å)