1Q7F

Brain Tumor NHL domain

Summary for 1Q7F

• Entry DOI: 10.2210/pdb1q7f/pdb
• Descriptor: brain tumor CG10719-PA (2 entities in total)
• Functional Keywords: brat, nhl domain, nhl repeat, beta-propeller, translation
• Biological source: Drosophila melanogaster (fruit fly)
• Cellular location: Cytoplasm : Q8MQJ9
• Total number of polymer chains: 2
• Total formula weight: 64843.85

Authors

Edwards, T.A.,Wilkinson, B.D.,Wharton, R.P.,Aggarwal, A.K. (deposition date: 2003-08-18, release date: 2003-10-21, Last modification date: 2024-02-14)

Primary citation

Edwards, T.A.,Wilkinson, B.D.,Wharton, R.P.,Aggarwal, A.K.
Model of the Brain Tumor-Pumilio translation repressor complex
Genes Dev., 17:2508-2513, 2003

PubMed Abstract: The Brain Tumor (Brat) protein is recruited to the 3' untranslated region (UTR) of hunchback mRNA to regulate its translation. Recruitment is mediated by interactions between the Pumilio RNA-binding Puf repeats and the NHL domain of Brat, a conserved structural motif present in a large family of growth regulators. In this report, we describe the crystal structure of the Brat NHL domain and present a model of the Pumilio-Brat complex derived from in silico docking experiments and supported by mutational analysis of the protein-protein interface. A key feature of the model is recognition of the outer, convex surface of the Pumilio Puf domain by the top, electropositive face of the six-bladed Brat beta-propeller. In particular, an extended loop in Puf repeat 8 fits in the entrance to the central channel of the Brat beta-propeller. Together, these interactions are likely to be prototypic of the recruitment strategies of other NHL-containing proteins in development.

PubMed: 14561773
DOI: 10.1101/gad.1119403

Experimental method

X-RAY DIFFRACTION (1.95 Å)