1Q3I

Crystal Structure of Na,K-ATPase N-domain

1Q3I の概要

• エントリーDOI: 10.2210/pdb1q3i/pdb
• 分子名称: Na,K-ATPase, NICKEL (II) ION (3 entities in total)
• 機能のキーワード: anti-parallel beta sheet, hydrolase
• 由来する生物種: Sus scrofa (pig)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24304.45

構造登録者

Hakansson, K.O. (登録日: 2003-07-30, 公開日: 2004-08-03, 最終更新日: 2024-02-14)

主引用文献

Hakansson, K.O.
The Crystallographic Structure of Na,K-ATPase N-domain at 2.6 A Resolution
J.Mol.Biol., 332:1175-1182, 2003

PubMed Abstract: The structure of the N-domain of porcine alpha(2) Na,K-ATPase was determined crystallographically to 3.2A resolution by isomorphous heavy-atom replacement using a single mercury derivative. The structure was finally refined against 2.6A resolution synchrotron data. The domain forms a seven-stranded antiparallel beta-sheet with two additional beta-strands forming a hairpin and five alpha-helices. Approximately 75% of the residues were superimposable with residues from the structure of Ca-ATPase N-domain, and a structure-based sequence alignment is presented. The positions of key residues are discussed in relation to the pattern of hydrophobicity, charge and sequence conservation of the molecular surface. The structure of a hexahistidine tag binding to nickel ions is presented.

PubMed: 14499619
DOI: 10.1016/j.jmb.2003.07.012

実験手法

X-RAY DIFFRACTION (2.6 Å)