1PXT

THE 2.8 ANGSTROMS STRUCTURE OF PEROXISOMAL 3-KETOACYL-COA THIOLASE OF SACCHAROMYCES CEREVISIAE: A FIVE LAYERED A-B-A-B-A STRUCTURE, CONSTRUCTED FROM TWO CORE DOMAINS OF IDENTICAL TOPOLOGY

1PXT の概要

• エントリーDOI: 10.2210/pdb1pxt/pdb
• 分子名称: PEROXISOMAL 3-KETOACYL-COA THIOLASE (1 entity in total)
• 機能のキーワード: thiolase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Peroxisome: P27796
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 83491.43

構造登録者

Mathieu, M.,Wierenga, R.K. (登録日: 1994-07-04, 公開日: 1994-08-31, 最終更新日: 2024-02-14)

主引用文献

Mathieu, M.,Zeelen, J.P.,Pauptit, R.A.,Erdmann, R.,Kunau, W.H.,Wierenga, R.K.
The 2.8 A crystal structure of peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: a five-layered alpha beta alpha beta alpha structure constructed from two core domains of identical topology.
Structure, 2:797-808, 1994

PubMed Abstract: The peroxisomal enzyme 3-ketoacyl-coenzyme A thiolase of the yeast Saccharomyces cerevisiae is a homodimer with 417 residues per subunit. It is synthesized in the cytosol and subsequently imported into the peroxisome where it catalyzes the last step of the beta-oxidation pathway. We have determined the structure of this thiolase in order to study the reaction mechanism, quaternary associations and intracellular targeting of thiolases generally, and to understand the structural basis of genetic disorders associated with human thiolases.

PubMed: 7812714
DOI: 10.1016/S0969-2126(94)00081-6

実験手法

X-RAY DIFFRACTION (2.8 Å)