1PVS

3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex

Summary for 1PVS

Entry DOI	10.2210/pdb1pvs/pdb
Descriptor	DNA-3-methyladenine glycosylase II, 7-HYDROXY-PYRAZOLO[4,3-D]PYRIMIDINE (3 entities in total)
Functional Keywords	alka, hypoxanthine, dna glycosylase, dna repair, reaction-product complex, hydrolase
Biological source	Escherichia coli
Total number of polymer chains	2
Total formula weight	63122.69
Authors	Teale, M. (deposition date: 2003-06-28, release date: 2004-06-08, Last modification date: 2024-02-14)
Primary citation	Teale, M.,Symersky, J.,DeLucas, L. 3-methyladenine-DNA glycosylase II: the crystal structure of an AlkA-hypoxanthine complex suggests the possibility of product inhibition. Bioconjug.Chem., 13:403-407, 2002 Cited by PubMed Abstract: Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II (AlkA) functions primarily by removing alkylation damage from duplex and single stranded DNA. A crystal structure of AlkA was refined to 2.0 A resolution. This structure in turn was used to refine an AlkA-hypoxanthine (substrate) complex structure to 2.4 A resolution. The complex structure shows hypoxanthine located in AlkA's active site stacked between residues W218 and Y239. The structural analysis of the AlkA and AlkA-hypoxanthine structures indicate that free hypoxanthine binding in the active site may inhibit glycosylase activity. PubMed: 12009927 DOI: 10.1021/bc015527v PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.4 Å)

Structure validation