1PVS

3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex

1PVS の概要

• エントリーDOI: 10.2210/pdb1pvs/pdb
• 分子名称: DNA-3-methyladenine glycosylase II, 7-HYDROXY-PYRAZOLO[4,3-D]PYRIMIDINE (3 entities in total)
• 機能のキーワード: alka, hypoxanthine, dna glycosylase, dna repair, reaction-product complex, hydrolase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 63122.69

構造登録者

Teale, M. (登録日: 2003-06-28, 公開日: 2004-06-08, 最終更新日: 2024-02-14)

主引用文献

Teale, M.,Symersky, J.,DeLucas, L.
3-methyladenine-DNA glycosylase II: the crystal structure of an AlkA-hypoxanthine complex suggests the possibility of product inhibition.
Bioconjug.Chem., 13:403-407, 2002

PubMed Abstract: Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II (AlkA) functions primarily by removing alkylation damage from duplex and single stranded DNA. A crystal structure of AlkA was refined to 2.0 A resolution. This structure in turn was used to refine an AlkA-hypoxanthine (substrate) complex structure to 2.4 A resolution. The complex structure shows hypoxanthine located in AlkA's active site stacked between residues W218 and Y239. The structural analysis of the AlkA and AlkA-hypoxanthine structures indicate that free hypoxanthine binding in the active site may inhibit glycosylase activity.

PubMed: 12009927
DOI: 10.1021/bc015527v

実験手法

X-RAY DIFFRACTION (2.4 Å)