1PUE

PU.1 ETS DOMAIN-DNA COMPLEX

1PUE の概要

• エントリーDOI: 10.2210/pdb1pue/pdb
• 分子名称: DNA (5'-D(*AP*AP*AP*AP*AP*GP*GP*GP*GP*AP*AP*GP*TP*GP*GP*G)-3'), DNA (5'-D(*TP*CP*CP*CP*AP*CP*TP*TP*CP*CP*CP*CP*TP*TP*TP*T)-3'), PROTEIN (TRANSCRIPTION FACTOR PU.1 (TF PU.1)), ... (4 entities in total)
• 機能のキーワード: complex (transcription regulating-dna), oncogene, transforming protein, dna- binding, activator, nuclear protein, transcription-dna complex, transcription/dna
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Nucleus: P17433
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 40809.70

構造登録者

Kodandapani, R.,Pio, F.,Ni, C.Z.,Piccialli, G.,Klemsz, M.,McKercher, S.,Maki, R.A.,Ely, K.R. (登録日: 1996-07-08, 公開日: 1997-02-12, 最終更新日: 2024-02-14)

主引用文献

Kodandapani, R.,Pio, F.,Ni, C.Z.,Piccialli, G.,Klemsz, M.,McKercher, S.,Maki, R.A.,Ely, K.R.
A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex.
Nature, 380:456-460, 1996

PubMed Abstract: The Ets family of transcription factors, of which there are now about 35 members regulate gene expression during growth and development. They share a conserved domain of around 85 amino acids which binds as a monomer to the DNA sequence 5'-C/AGGAA/T-3'. We have determined the crystal structure of an ETS domain complexed with DNA, at 2.3-A resolution. The domain is similar to alpha + beta (winged) 'helix-turn-helix' proteins and interacts with a ten-base-pair region of duplex DNA which takes up a uniform curve of 8 degrees. The domain contacts the DNA by a novel loop-helix-loop architecture. Four of amino acids that directly interact with the DNA are highly conserved: two arginines from the recognition helix lying in the major groove, one lysine from the 'wing' that binds upstream of the core GGAA sequence, and another lysine, from the 'turn' of the 'helix-turn-helix' motif, which binds downstream and on the opposite strand.

PubMed: 8602247
DOI: 10.1038/380456a0

実験手法

X-RAY DIFFRACTION (2.1 Å)