1PTO

THE STRUCTURE OF A PERTUSSIS TOXIN-SUGAR COMPLEX AS A MODEL FOR RECEPTOR BINDING

1PTO の概要

• エントリーDOI: 10.2210/pdb1pto/pdb
• 分子名称: PERTUSSIS TOXIN (SUBUNIT S1), PERTUSSIS TOXIN, PERTUSSIS TOXIN (SUBUNIT S4), ... (7 entities in total)
• 機能のキーワード: toxin
• 由来する生物種: Bordetella pertussis; 詳細
• 細胞内の位置: Secreted: P04978 P04979 P04980 P04981 P04978
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 212512.46

構造登録者

Stein, P.E.,Read, R.J. (登録日: 1994-03-22, 公開日: 1995-09-15, 最終更新日: 2024-10-30)

主引用文献

Stein, P.E.,Boodhoo, A.,Armstrong, G.D.,Heerze, L.D.,Cockle, S.A.,Klein, M.H.,Read, R.J.
Structure of a pertussis toxin-sugar complex as a model for receptor binding.
Nat.Struct.Biol., 1:591-596, 1994

PubMed Abstract: Pertussis toxin is an exotoxin from the bacterium Bordetella pertussis which is important the pathogenesis of whooping cough and the generation of a protective immune response. The diverse biological activities of the toxin depend on its ability to recognize carbohydrate-containing receptors on a wide variety of eukaryotic cells. We present here the crystal structure of pertussis toxin complexed with a soluble oligosaccharide from transferrin. Binding sites for the terminal sialic acid-galactose moiety are revealed on both subunits S2 and S3 of the B-oligomer. Identification of amino acid residues involved in receptor binding will improve the design of genetically inactivated toxins for use in new acellular whooping cough vaccines.

PubMed: 7634099
DOI: 10.1038/nsb0994-591

実験手法

X-RAY DIFFRACTION (3.5 Å)