1PP8

crystal structure of the T. vaginalis IBP39 Initiator binding domain (IBD) bound to the alpha-SCS Inr element

1PP8 の概要

• エントリーDOI: 10.2210/pdb1pp8/pdb
• 関連するPDBエントリー: 1PP7
• 分子名称: ALPHA-SCS INR, 39 kDa initiator binding protein, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: ibp39, initiator binding protein, inr, core promoter, transcription, t. vaginalis, transcription-dna complex, transcription/dna
• 由来する生物種: Trichomonas vaginalis
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 121553.75

構造登録者

Schumacher, M.A.,Lau, A.O.T.,Johnson, P.J. (登録日: 2003-06-16, 公開日: 2003-11-18, 最終更新日: 2024-02-14)

主引用文献

Schumacher, M.A.,Lau, A.O.T.,Johnson, P.J.
Structural Basis of Core Promoter Recognition in a Primitive Eukaryote
Cell(Cambridge,Mass.), 115:413-424, 2003

PubMed Abstract: Transcription start site selection in eukaryotes is mediated through combinations of the TATA, initiator (Inr), and downstream promoter elements (DPE). In Trichomonas vaginalis, a parabasalian flagellate thought to represent an ancient eukaryote lineage, the Inr appears to be solely responsible for start site selection and is recognized by the initiator binding protein 39 kDa (IBP39). IBP39 contains an N-terminal Inr binding domain (IBD) connected via a flexible linker to a C-terminal domain (C domain). Here we present crystal structures of the apoIBD and IBD-Inr complexes and the C domain. The IBD structures reveal a winged-helix motif with prokaryotic and eukaryotic features and a scaffold similar to that of ETS-family proteins. The C domain structure and biochemical studies indicate that it interacts with the T. vaginalis RNAP II large subunit C-terminal domain. These data suggest that binding of IBP39 to the Inr directly recruits RNAP II and in this way initiates transcription.

PubMed: 14622596
DOI: 10.1016/S0092-8674(03)00887-0

実験手法

X-RAY DIFFRACTION (3.05 Å)