1POB

CRYSTAL STRUCTURE OF COBRA-VENOM PHOSPHOLIPASE A2 IN A COMPLEX WITH A TRANSITION-STATE ANALOGUE

1POB の概要

• エントリーDOI: 10.2210/pdb1pob/pdb
• 分子名称: PHOSPHOLIPASE A2, CALCIUM ION, 1-O-OCTYL-2-HEPTYLPHOSPHONYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE, ... (4 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Naja atra (Chinese cobra)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27448.64

構造登録者

White, S.P.,Scott, D.L.,Otwinowski, Z.,Sigler, P.B. (登録日: 1992-09-07, 公開日: 1993-10-31, 最終更新日: 2024-12-25)

主引用文献

White, S.P.,Scott, D.L.,Otwinowski, Z.,Gelb, M.H.,Sigler, P.B.
Crystal structure of cobra-venom phospholipase A2 in a complex with a transition-state analogue.
Science, 250:1560-1563, 1990

PubMed Abstract: The crystal structure of a complex between a phosphonate transition-state analogue and the phospholipase A2 (PLA2) from Naja naja atra venom has been solved and refined to a resolution of 2.0 angstroms. The identical stereochemistry of the two complexes that comprise the crystal's asymmetric unit indicates both the manner in which the transition state is stabilized and how the hydrophobic fatty acyl chains of the substrate are accommodated by the enzyme during interfacial catalysis. The critical features that suggest the chemistry of binding and catalysis are the same as those seen in the crystal structure of a similar complex formed with the evolutionarily distant bee-venom PLA2.

PubMed: 2274787

実験手法

X-RAY DIFFRACTION (2 Å)