1PH9

CRYSTAL STRUCTURE OF THE OXYTRICHA NOVA TELOMERE END-BINDING PROTEIN COMPLEXED WITH NONCOGNATE SSDNA GGGGTTTTGAGG

1PH9 の概要

• エントリーDOI: 10.2210/pdb1ph9/pdb
• 関連するPDBエントリー: 1jb7 1k8g 1kix 1otc 1pa6 1ph1 1ph2 1ph3 1ph4 1ph5 1ph6 1ph7 1ph8 1phj
• 分子名称: 5'-D(*GP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*GP*T)-3', 5'-D(*GP*GP*GP*GP*TP*TP*TP*TP*GP*AP*GP*G)-3', Telomere-binding protein alpha subunit, ... (7 entities in total)
• 機能のキーワード: single strand dna binding protein, protein dna interactions, sequence specificity, noncognate, oligonucleotide and oligosaccharide binding fold, ob fold, telomeres, protein/dna, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Sterkiella nova; 詳細
• 細胞内の位置: Nucleus: P29549 P16458
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 89083.89

構造登録者

Theobald, D.L.,Schultz, S.C. (登録日: 2003-05-29, 公開日: 2003-06-17, 最終更新日: 2024-02-14)

主引用文献

Theobald, D.L.,Schultz, S.C.
Nucleotide Shuffling and ssDNA Recognition in Oxytricha Nova Telomere End-Binding Protein Complexes
Embo J., 22:4314-4324, 2003

PubMed Abstract: Sequence-specific protein recognition of single-stranded nucleic acids is critical for many fundamental cellular processes, such as DNA replication, DNA repair, transcription, translation, recombination, apoptosis and telomere maintenance. To explore the mechanisms of sequence-specific ssDNA recognition, we determined the crystal structures of 10 different non-cognate ssDNAs complexed with the Oxytricha nova telomere end-binding protein (OnTEBP) and evaluated their corresponding binding affinities (PDB ID codes 1PH1-1PH9 and 1PHJ). The thermodynamic and structural effects of these sequence perturbations could not have been predicted based solely upon the cognate structure. OnTEBP accommodates non-cognate nucleotides by both subtle adjustments and surprisingly large structural rearrangements in the ssDNA. In two complexes containing ssDNA intermediates that occur during telomere extension by telomerase, entire nucleotides are expelled from the complex. Concurrently, the sequence register of the ssDNA shifts to re-establish a more cognate-like pattern. This phenomenon, termed nucleotide shuffling, may be of general importance in protein recognition of single-stranded nucleic acids. This set of structural and thermodynamic data highlights a fundamental difference between protein recognition of ssDNA versus dsDNA.

PubMed: 12912928
DOI: 10.1093/emboj/cdg415

実験手法

X-RAY DIFFRACTION (2.5 Å)