1PEY

Crystal structure of the Response Regulator Spo0F complexed with Mn2+

1PEY の概要

• エントリーDOI: 10.2210/pdb1pey/pdb
• 関連するPDBエントリー: 1NAT 1SRR
• 分子名称: Sporulation initiation phosphotransferase F, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: cation binding, (alpha/beta) protein, transferase
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 42670.51

構造登録者

Mukhopadhyay, D.,Sen, U.,Zapf, J.,Varughese, K.I. (登録日: 2003-05-22, 公開日: 2004-05-18, 最終更新日: 2024-02-14)

主引用文献

Mukhopadhyay, D.,Sen, U.,Zapf, J.,Varughese, K.I.
Metals in the sporulation phosphorelay: manganese binding by the response regulator Spo0F.
Acta Crystallogr.,Sect.D, 60:638-645, 2004

PubMed Abstract: As a part of studies on the structural characterization of the components of the sporulation phosphorelay in Bacillus subtilis, the crystal structure of the manganese derivative of an intermediate signal transducer, Spo0F, has been elucidated at 2.25 A resolution. The calcium complex and the apo structures have been analyzed previously. In apo Spo0F, the active-site cation cavity is only partially formed and it only becomes completed upon metal coordination. The carbonyl of Lys56 is coordinated to the metal and interestingly the side chain of Lys56 exists in a variety of conformations in the three crystal structures of Spo0F. The affinity of the magnesium ion for Spo0F is in fact low; however, it binds Spo0F when it is in complex with Spo0B. It is proposed that the existence of a deep pocket which extends from the surface to the metal site could attract and direct the metal, thereby facilitating the metal binding of the complex.

PubMed: 15039551
DOI: 10.1107/S0907444904002148

実験手法

X-RAY DIFFRACTION (2.25 Å)