1PD2

CRYSTAL STRUCTURE OF HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE COMPLEX WITH GLUTATHIONE

1PD2 の概要

• エントリーDOI: 10.2210/pdb1pd2/pdb
• 分子名称: HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE, GLUTATHIONE (3 entities in total)
• 機能のキーワード: hematopoietic prostaglandin d synthase, pgds, gst, sigma-class gst, ligase
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Cytoplasm: O35543
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47262.53

構造登録者

Miyano, M.,Ago, H. (登録日: 1998-12-14, 公開日: 1999-10-13, 最終更新日: 2023-12-27)

主引用文献

Kanaoka, Y.,Ago, H.,Inagaki, E.,Nanayama, T.,Miyano, M.,Kikuno, R.,Fujii, Y.,Eguchi, N.,Toh, H.,Urade, Y.,Hayaishi, O.
Cloning and crystal structure of hematopoietic prostaglandin D synthase.
Cell(Cambridge,Mass.), 90:1085-1095, 1997

PubMed Abstract: Hematopoietic prostaglandin (PG) D synthase is the key enzyme for production of the D and J series of prostanoids in the immune system and mast cells. We isolated a cDNA for the rat enzyme, crystallized the recombinant enzyme, and determined the three-dimensional structure of the enzyme complexed with glutathione at 2.3 A resolution. The enzyme is the first member of the sigma class glutathione S-transferase (GST) from vertebrates and possesses a prominent cleft as the active site, which is never seen among other members of the GST family. The unique 3-D architecture of the cleft leads to the putative substrate binding mode and its catalytic mechanism, responsible for the specific isomerization from PGH2 to PGD2.

PubMed: 9323136
DOI: 10.1016/S0092-8674(00)80374-8

実験手法

X-RAY DIFFRACTION (2.3 Å)