1PCQ

Crystal structure of groEL-groES

1PCQ の概要

• エントリーDOI: 10.2210/pdb1pcq/pdb
• 分子名称: groEL protein, groES protein, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: chaperone
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm: P0A6F5 P0A6F9
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 849910.10

構造登録者

Chaudhry, C.,Farr, G.W.,Todd, M.J.,Rye, H.S.,Brunger, A.T.,Adams, P.D.,Horwich, A.L.,Sigler, P.B. (登録日: 2003-05-16, 公開日: 2003-10-14, 最終更新日: 2024-02-14)

主引用文献

Chaudhry, C.,Farr, G.W.,Todd, M.J.,Rye, H.S.,Brunger, A.T.,Adams, P.D.,Horwich, A.L.,Sigler, P.B.
Role of the gamma-phosphate of ATP in triggering protein folding by GroEL-GroES: function, structure and energetics.
Embo J., 22:4877-4887, 2003

PubMed Abstract: Productive cis folding by the chaperonin GroEL is triggered by the binding of ATP but not ADP, along with cochaperonin GroES, to the same ring as non-native polypeptide, ejecting polypeptide into an encapsulated hydrophilic chamber. We examined the specific contribution of the gamma-phosphate of ATP to this activation process using complexes of ADP and aluminium or beryllium fluoride. These ATP analogues supported productive cis folding of the substrate protein, rhodanese, even when added to already-formed, folding-inactive cis ADP ternary complexes, essentially introducing the gamma-phosphate of ATP in an independent step. Aluminium fluoride was observed to stabilize the association of GroES with GroEL, with a substantial release of free energy (-46 kcal/mol). To understand the basis of such activation and stabilization, a crystal structure of GroEL-GroES-ADP.AlF3 was determined at 2.8 A. A trigonal AlF3 metal complex was observed in the gamma-phosphate position of the nucleotide pocket of the cis ring. Surprisingly, when this structure was compared with that of the previously determined GroEL-GroES-ADP complex, no other differences were observed. We discuss the likely basis of the ability of gamma-phosphate binding to convert preformed GroEL-GroES-ADP-polypeptide complexes into the folding-active state.

PubMed: 14517228
DOI: 10.1093/emboj/cdg477

実験手法

X-RAY DIFFRACTION (2.808 Å)