1P65

Crystal structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus (PRRSV)

1P65 の概要

• エントリーDOI: 10.2210/pdb1p65/pdb
• 分子名称: nucleocapsid protein (2 entities in total)
• 機能のキーワード: virus, nucleocapsid, viral protein
• 由来する生物種: Porcine respiratory and reproductive syndrome virus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16344.06

構造登録者

Doan, D.N.P.,Dokland, T. (登録日: 2003-04-29, 公開日: 2003-11-25, 最終更新日: 2024-02-14)

主引用文献

Doan, D.N.P.,Dokland, T.
Structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus.
Structure, 11:1445-1451, 2003

PubMed Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped RNA virus of the Arteriviridae family, genomically related to the coronaviruses. PRRSV is the causative agent of both severe and persistent respiratory disease and reproductive failure in pigs worldwide. The PRRSV virion contains a core made of the 123 amino acid nucleocapsid (N) protein, a product of the ORF7 gene. We have determined the crystal structure of the capsid-forming domain of N. The structure was solved to 2.6 A resolution by SAD methods using the anomalous signal from sulfur. The N protein exists in the crystal as a tight dimer forming a four-stranded beta sheet floor superposed by two long alpha helices and flanked by two N- and two C-terminal alpha helices. The structure of N represents a new class of viral capsid-forming domains, distinctly different from those of other known enveloped viruses, but reminiscent of the coat protein of bacteriophage MS2.

PubMed: 14604534
DOI: 10.1016/j.str.2003.09.018

実験手法

X-RAY DIFFRACTION (2.6 Å)