1OZB

Crystal Structure of SecB complexed with SecA C-terminus

1OZB の概要

• エントリーDOI: 10.2210/pdb1ozb/pdb
• 関連するPDBエントリー: 1FX3 1M6N 1NL3
• 分子名称: Protein-export protein secB, Preprotein translocase secA subunit, ZINC ION (3 entities in total)
• 機能のキーワード: zinc binding motif, protein-protein complex, protein transport
• 由来する生物種: Haemophilus influenzae; 詳細
• 細胞内の位置: Cytoplasm: P44853; Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P43803
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 159370.29

構造登録者

Zhou, J.,Xu, Z. (登録日: 2003-04-08, 公開日: 2003-10-14, 最終更新日: 2024-04-03)

主引用文献

Zhou, J.,Xu, Z.
Structural determinants of SecB recognition by SecA in bacterial protein translocation
NAT.STRUCT.BIOL., 10:942-947, 2003

PubMed Abstract: SecB is a bacterial chaperone involved in directing pre-protein to the translocation pathway by its specific interaction with the peripheral membrane ATPase SecA. The SecB-binding site on SecA is located at its C terminus and consists of a stretch of highly conserved residues. The crystal structure of SecB in complex with the C-terminal 27 amino acids of SecA from Haemophilus influenzae shows that the SecA peptide is structured as a CCCH zinc-binding motif. One SecB tetramer is bound by two SecA peptides, and the interface involves primarily salt bridges and hydrogen bonding interactions. The structure explains the importance of the zinc-binding motif and conserved residues at the C terminus of SecA in its high-affinity binding with SecB. It also suggests a model of SecB-SecA interaction and its implication for the mechanism of pre-protein transfer in bacterial protein translocation.

PubMed: 14517549
DOI: 10.1038/nsb980

実験手法

X-RAY DIFFRACTION (2.8 Å)