1OYU

Long-Distance conformational changes in a protein engineered by modulated sequence duplication

Summary for 1OYU

• Entry DOI: 10.2210/pdb1oyu/pdb
• Related: 261L 262L
• Descriptor: Lysozyme (2 entities in total)
• Functional Keywords: sequence duplication, design of structural switches, tandem repeat, protein design, hydrolase
• Biological source: Enterobacteria phage T4
• Total number of polymer chains: 2
• Total formula weight: 39026.72

Authors

Sagermann, M.,Gay, L.,Matthews, B.W. (deposition date: 2003-04-07, release date: 2003-07-08, Last modification date: 2023-08-16)

Primary citation

Sagermann, M.,Gay, L.,Matthews, B.W.
Long-distance conformational changes in a protein engineered by modulated sequence duplication
Proc.Natl.Acad.Sci.USA, 100:9191-9195, 2003

PubMed Abstract: There are few, if any, known instances in which a biological signal is transmitted via a large conformational change through the body of a protein. We describe here a mutant of T4 lysozyme that was engineered to permit structural change at a distance. The design uses a tandem sequence repeat that makes it possible to transmit large-scale structural changes from one end of an alpha-helix to the other over a distance of 17-25 A. The method should be of general applicability and may make it possible to introduce a mutation at one site in a protein that will induce large-scale changes in the structure at a spatially remote site.

PubMed: 12869697
DOI: 10.1073/pnas.1633549100

Experimental method

X-RAY DIFFRACTION (2.5 Å)