1OYU

Long-Distance conformational changes in a protein engineered by modulated sequence duplication

1OYU の概要

• エントリーDOI: 10.2210/pdb1oyu/pdb
• 関連するPDBエントリー: 261L 262L
• 分子名称: Lysozyme (2 entities in total)
• 機能のキーワード: sequence duplication, design of structural switches, tandem repeat, protein design, hydrolase
• 由来する生物種: Enterobacteria phage T4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39026.72

構造登録者

Sagermann, M.,Gay, L.,Matthews, B.W. (登録日: 2003-04-07, 公開日: 2003-07-08, 最終更新日: 2023-08-16)

主引用文献

Sagermann, M.,Gay, L.,Matthews, B.W.
Long-distance conformational changes in a protein engineered by modulated sequence duplication
Proc.Natl.Acad.Sci.USA, 100:9191-9195, 2003

PubMed Abstract: There are few, if any, known instances in which a biological signal is transmitted via a large conformational change through the body of a protein. We describe here a mutant of T4 lysozyme that was engineered to permit structural change at a distance. The design uses a tandem sequence repeat that makes it possible to transmit large-scale structural changes from one end of an alpha-helix to the other over a distance of 17-25 A. The method should be of general applicability and may make it possible to introduce a mutation at one site in a protein that will induce large-scale changes in the structure at a spatially remote site.

PubMed: 12869697
DOI: 10.1073/pnas.1633549100

実験手法

X-RAY DIFFRACTION (2.5 Å)