1OXB

Complex between YPD1 and SLN1 response regulator domain in space group P2(1)2(1)2(1)

1OXB の概要

• エントリーDOI: 10.2210/pdb1oxb/pdb
• 関連するPDBエントリー: 1OXK 1QSP
• 分子名称: Ypd1p, SLN1, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: phosphorelay protein, two-component signaling protein, response regulator, hpt domain, histidine-containing phosphotransfer protein, ypd1p, sln1p, signaling protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Cytoplasm: Q07688; Cell membrane; Multi-pass membrane protein (Potential): P39928
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34245.04

構造登録者

Xu, Q.,Porter, S.W.,West, A.H. (登録日: 2003-04-01, 公開日: 2003-12-16, 最終更新日: 2023-08-16)

主引用文献

Xu, Q.,Porter, S.W.,West, A.H.
The yeast YPD1/SLN1 complex: insights into molecular recognition in two-component signaling systems.
Structure, 11:1569-1581, 2003

PubMed Abstract: In Saccharomyces cerevisiae, a branched multistep phosphorelay signaling pathway regulates cellular adaptation to hyperosmotic stress. YPD1 functions as a histidine-phosphorylated protein intermediate required for phosphoryl group transfer from a membrane-bound sensor histidine kinase (SLN1) to two distinct response regulator proteins (SSK1 and SKN7). These four proteins are evolutionarily related to the well-characterized "two-component" regulatory proteins from bacteria. Although structural information is available for many two-component signaling proteins, there are very few examples of complexes between interacting phosphorelay partners. Here we report the first crystal structure of a prototypical monomeric histidine-containing phosphotransfer (HPt) protein YPD1 in complex with its upstream phosphodonor, the response regulator domain associated with SLN1.

PubMed: 14656441
DOI: 10.1016/j.str.2003.10.016

実験手法

X-RAY DIFFRACTION (2.3 Å)