1OXA

CYTOCHROME P450 (DONOR:O2 OXIDOREDUCTASE)

1OXA の概要

• エントリーDOI: 10.2210/pdb1oxa/pdb
• 分子名称: CYTOCHROME P450 ERYF, PROTOPORPHYRIN IX CONTAINING FE, 6-DEOXYERYTHRONOLIDE B, ... (4 entities in total)
• 機能のキーワード: oxidoreductase (oxygenase)
• 由来する生物種: Saccharopolyspora erythraea
• 細胞内の位置: Cytoplasm (By similarity): Q00441
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45518.16

構造登録者

Cupp-Vickery, J.R.,Poulos, T.L. (登録日: 1995-07-14, 公開日: 1995-12-07, 最終更新日: 2024-02-14)

主引用文献

Cupp-Vickery, J.R.,Poulos, T.L.
Structure of cytochrome P450eryF involved in erythromycin biosynthesis.
Nat.Struct.Biol., 2:144-153, 1995

PubMed Abstract: Cytochrome P450eryF catalyzes the 6S-hydroxylation of 6-deoxyerythronolide B, the initial reaction in a multistep pathway to convert 6-deoxyerythronolide B into the antibiotic, erythromycin. The overall structure of P450eryF is similar to that of P450cam but differs in the exact positioning of several alpha-helices. The largest difference occurs in the B' helix and results in the enlargement of the substrate-binding pocket of P450eryF. The substrate is positioned with the macrolide ring perpendicular to the haem plane and contacts seven hydrophobic residues and three solvent molecules. The substrate participates in a network of hydrogen bonds that may provide a proton shuttle pathway in the oxygen cleavage reaction.

PubMed: 7749919
DOI: 10.1038/nsb0295-144

実験手法

X-RAY DIFFRACTION (2.1 Å)