1OX9

Crystal structure of SspB-ssrA complex

1OX9 の概要

• エントリーDOI: 10.2210/pdb1ox9/pdb
• 関連するPDBエントリー: 1OX8
• 分子名称: Stringent starvation protein B, ssrA (2 entities in total)
• 機能のキーワード: sspb-ssra, hydrolase activator
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 103083.98

構造登録者

Song, H.K.,Eck, M.J. (登録日: 2003-04-01, 公開日: 2003-08-26, 最終更新日: 2024-02-14)

主引用文献

Song, H.K.,Eck, M.J.
Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine
Mol.Cell, 12:75-86, 2003

PubMed Abstract: In prokaryotes, incomplete or misfolded polypeptides emanating from a stalled ribosome are marked for degradation by the addition of an 11 residue peptide (AANDENYALAA) to their C terminus. Substrates containing this conserved degradation signal, the SsrA tag, are targeted to specific proteases including ClpXP and ClpAP. SspB was originally characterized as a stringent starvation protein and has been found to bind specifically to SsrA-tagged proteins and to enhance recognition of these proteins by the ClpXP degradation machine. Here, we report the crystal structures of SspB alone and in complex with an SsrA peptide. Unexpectedly, SspB exhibits a fold found in Sm-family RNA binding proteins. The dimeric SspB structures explain the key determinants for recognition of the SsrA tag and define a hydrophobic channel that may bind unfolded substrates.

PubMed: 12887894
DOI: 10.1016/S1097-2765(03)00271-5

実験手法

X-RAY DIFFRACTION (2.9 Å)