1OWF

Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site

1OWF の概要

• エントリーDOI: 10.2210/pdb1owf/pdb
• 関連するPDBエントリー: 1IHF 1OUZ 1OWG
• 分子名称: Phage lambda H' site, 5'-D(*GP*GP*CP*CP*AP*AP*AP*AP*AP*AP*GP*CP*AP*TP*T)-3', 5'-D(*GP*CP*TP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*CP*AP*CP*C)-3', ... (6 entities in total)
• 機能のキーワード: protein-dna recognition, indirect readout, ihf, dna bending, minor groove, transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 43475.04

構造登録者

Lynch, T.W.,Read, E.K.,Mattis, A.N.,Gardner, J.F.,Rice, P.A. (登録日: 2003-03-28, 公開日: 2003-07-15, 最終更新日: 2024-02-14)

主引用文献

Lynch, T.W.,Read, E.K.,Mattis, A.N.,Gardner, J.F.,Rice, P.A.
Integration Host Factor: putting a twist on protein-DNA recognition
J.Mol.Biol., 330:493-502, 2003

PubMed Abstract: Integration host factor (IHF) is a DNA-bending protein that recognizes its cognate sites through indirect readout. Previous studies have shown that binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the center position of a conserved TTR motif in its binding site, and that substitution of betaGlu44 with Ala prevented IHF from discriminating between A and T at this position. We have determined the crystal structures and relative binding affinities for all combinations of WT-IHF and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these structures reveals that DNA twist plays a major role in DNA recognition by IHF, and that this geometric parameter is dependent on the dinucleotide step and not on the bound IHF variant.

PubMed: 12842466
DOI: 10.1016/S0022-2836(03)00529-1

実験手法

X-RAY DIFFRACTION (1.95 Å)