1OTC

THE O. NOVA TELOMERE END BINDING PROTEIN COMPLEXED WITH SINGLE STRAND DNA

1OTC の概要

• エントリーDOI: 10.2210/pdb1otc/pdb
• 分子名称: DNA (5'-D(*GP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*G)-3'), PROTEIN (TELOMERE-BINDING PROTEIN ALPHA SUBUNIT), PROTEIN (TELOMERE-BINDING PROTEIN BETA SUBUNIT), ... (4 entities in total)
• 機能のキーワード: single strand dna binding protein, protein dna interactions, protein protein interactions, oligonucleotide and oligosaccharide binding fold, ob fold, telomeres, protein-dna complex, protein/dna
• 由来する生物種: Sterkiella nova; 詳細
• 細胞内の位置: Nucleus: P29549 P16458
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 88690.43

構造登録者

Horvath, M.P.,Schweiker, V.L.,Bevilacqua, J.M.,Ruggles, J.A.,Schultz, S.C. (登録日: 1998-11-25, 公開日: 1999-04-12, 最終更新日: 2023-12-27)

主引用文献

Horvath, M.P.,Schweiker, V.L.,Bevilacqua, J.M.,Ruggles, J.A.,Schultz, S.C.
Crystal structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA.
Cell(Cambridge,Mass.), 95:963-974, 1998

PubMed Abstract: Telomeres are specialized protein-DNA complexes that compose the ends of eukaryotic chromosomes. Telomeres protect chromosome termini from degradation and recombination and act together with telomerase to ensure complete genome replication. We have determined the crystal structure of the two-subunit Oxytricha nova telomere end binding protein (OnTEBP) complexed with single strand telomeric DNA at 2.8 A resolution. The structure reveals four oligonucleotide/oligosaccharide-binding folds, three of which form a deep cleft that binds the ssDNA, and a fourth that forms an unusual protein-protein interaction between the alpha and beta subunits. This structure provides a molecular description of how the two subunits of OnTEBP recognize and bind ssDNA to form a sequence-specific, telomeric nucleoprotein complex that caps the very 3' ends of chromosomes.

PubMed: 9875850
DOI: 10.1016/S0092-8674(00)81720-1

実験手法

X-RAY DIFFRACTION (2.8 Å)