1OSD

crystal structure of Oxidized MerP from Ralstonia metallidurans CH34

1OSD の概要

• エントリーDOI: 10.2210/pdb1osd/pdb
• 関連するPDBエントリー: 1AFI 1AFJ 2HQI
• 分子名称: hypothetical protein MerP (2 entities in total)
• 機能のキーワード: mercury resistance, metal binding protein, perisplasm, structural genomics
• 由来する生物種: Cupriavidus metallidurans
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15061.24

構造登録者

Serre, L.,Rossy, E.,Pebay-Peyroula, E.,Cohen-Addad, C.,Coves, J. (登録日: 2003-03-19, 公開日: 2004-05-18, 最終更新日: 2024-10-16)

主引用文献

Serre, L.,Rossy, E.,Pebay-Peyroula, E.,Cohen-Addad, C.,Coves, J.
Crystal Structure of the Oxidized Form of the Periplasmic Mercury-binding Protein MerP from Ralstonia metallidurans CH34
J.MOL.BIOL., 339:161-171, 2004

PubMed Abstract: In Ralstonia metallidurans CH34, the gene merP encodes for a periplasmic mercury-binding protein which is capable of binding one mercury atom. The metal-binding site of MerP consists of the highly conserved sequence GMTCXXC found in the family that includes metallochaperones and metal-transporting ATPases. We purified MerP from R.metallidurans CH34 and solved its crystal structure under the oxidized form at 2.0A resolution. Superposition with structures of other metal-binding proteins shows that the global structure of R.metallidurans CH34 oxidized MerP follows the general topology of the whole family. The largest differences are observed with the NMR structure of oxidized Shigella flexneri MerP. Detailed analysis of the metal-binding site suggests a direct role for Y66 in stabilizing the thiolate group of C17 during the mercury-binding reaction. The metal-binding site of oxidized MerP is also similar to the metal-binding sites of oxidized copper chaperone for superoxide dismutase and Atx1, two copper-binding proteins from Saccharomyces cerevisiae. Finally, the packing of the MerP crystals suggests that F38, a well-conserved residue in the MerP family may be important in mercury binding and transfer. We propose a possible mechanism of mercury transfer between two CXXC motifs based on a transient bi-coordinated mercury intermediate.

PubMed: 15123428
DOI: 10.1016/j.jmb.2004.03.022

実験手法

X-RAY DIFFRACTION (2 Å)