1ORC
CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]
Summary for 1ORC
| Entry DOI | 10.2210/pdb1orc/pdb |
| Descriptor | CRO REPRESSOR INSERTION MUTANT K56-[DGEVK] (2 entities in total) |
| Functional Keywords | gene regulating protein |
| Biological source | Enterobacteria phage lambda |
| Total number of polymer chains | 1 |
| Total formula weight | 7905.05 |
| Authors | Albright, R.A.,Mossing, M.C.,Matthews, B.W. (deposition date: 1995-10-30, release date: 1996-12-23, Last modification date: 2024-02-14) |
| Primary citation | Albright, R.A.,Mossing, M.C.,Matthews, B.W. High-resolution structure of an engineered Cro monomer shows changes in conformation relative to the native dimer. Biochemistry, 35:735-742, 1996 Cited by PubMed Abstract: A rationally designed, genetically engineered, monomeric form of the Cro protein from bacteriophage lambda has been crystallized and its structure determined by isomorphous replacement and refined to a resolution of 1.54 A. The structure confirms the rationale of the design but, at the same time, reveals 1-2 A shifts throughout the monomer structure relative to the previously determined structure of the dimeric wild-type protein. These changes include a 1.6 A main-chain shift in part of the beta-sheet region of the molecule relative to the alpha-helical region and a 1.1 A shift of a buried phenylalanine within the core as well as a correlated 2.2 A shift in a solvent-exposed beta-hairpin. The conformational adjustments appear to reflect an inherent flexibility of the protein that is associated with its DNA-binding function. PubMed: 8547253DOI: 10.1021/bi951958n PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.54 Å) |
Structure validation
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