1OR7

Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA

1OR7 の概要

• エントリーDOI: 10.2210/pdb1or7/pdb
• 分子名称: RNA polymerase sigma-E factor, Sigma-E factor negative regulatory protein (3 entities in total)
• 機能のキーワード: regulation, dna-binding, transmembrane, transcription
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cell membrane; Single-pass membrane protein (Potential): P0AFX7
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64825.41

構造登録者

Campbell, E.A.,Tupy, J.L.,Gruber, T.M.,Wang, S.,Sharp, M.M.,Gross, C.A.,Darst, S.A. (登録日: 2003-03-12, 公開日: 2003-04-15, 最終更新日: 2024-02-14)

主引用文献

Campbell, E.A.,Tupy, J.L.,Gruber, T.M.,Wang, S.,Sharp, M.M.,Gross, C.A.,Darst, S.A.
Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA.
Mol.Cell, 11:1067-1078, 2003

PubMed Abstract: The sigma factors are the key regulators of bacterial transcription. ECF (extracytoplasmic function) sigma's are the largest and most divergent group of sigma(70) family members. ECF sigma's are normally sequestered in an inactive complex by their specific anti-sigma factor, which often spans the inner membrane. Here, we determined the 2 A resolution crystal structure of the Escherichia coli ECF sigma factor sigma(E) in an inhibitory complex with the cytoplasmic domain of its anti-sigma, RseA. Despite extensive sequence variability, the two major domains of sigma(E) are virtually identical in structure to the corresponding domains of other sigma(70) family members. In combination with a model of the sigma(E) holoenzyme and biochemical data, the structure reveals that RseA functions by sterically occluding the two primary binding determinants on sigma(E) for core RNA polymerase.

PubMed: 12718891
DOI: 10.1016/S1097-2765(03)00148-5

実験手法

X-RAY DIFFRACTION (2 Å)