1OQA

Solution structure of the BRCT-c domain from human BRCA1

1OQA の概要

• エントリーDOI: 10.2210/pdb1oqa/pdb
• 関連するPDBエントリー: 1CDZ 1JNX 1KZY
• NMR情報: BMRB: 6114
• 分子名称: Breast cancer type 1 susceptibility protein (1 entity in total)
• 機能のキーワード: brct, breast cancer, gene regulation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus. Isoform 3: Cytoplasm. Isoform 5: Cytoplasm: P38398
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12367.03

構造登録者

Gaiser, O.J.,Ball, L.J.,Schmieder, P.,Leitner, D.,Strauss, H.,Wahl, M.,Kuhne, R.,Oschkinat, H.,Heinemann, U. (登録日: 2003-03-07, 公開日: 2004-06-15, 最終更新日: 2024-05-22)

主引用文献

Gaiser, O.J.,Ball, L.J.,Schmieder, P.,Leitner, D.,Strauss, H.,Wahl, M.,Kuhne, R.,Oschkinat, H.,Heinemann, U.
Solution structure, backbone dynamics, and association behavior of the C-terminal BRCT domain from the breast cancer-associated protein BRCA1.
Biochemistry, 43:15983-15995, 2004

PubMed Abstract: BRCA1 is a tumor suppressor protein associated with breast and ovarian cancer. The C-terminal region of BRCA1 consists of two closely spaced BRCT domains which mediate essential biological functions, including regulation of transcription and control of cell-cycle progression by their interaction with phosphorylated effector proteins. Here we report the NMR structure of the isolated C-terminal BRCT domain (BRCT-c) from human BRCA1. BRCT-c is well-structured in solution, folding independently in the absence of its BRCT-n counterpart. Ultracentrifugation experiments and size exclusion chromatography reveal that BRCT-c exists as a monomer under near-physiological conditions. Dynamics measurements from NMR data show three loops which coincide with the most variable sequence regions in BRCT domains, to be genuinely flexible in solution. The solution structure of BRCT-c shows subtle conformational changes when compared to the crystal structure of BRCT-c in the tandem repeat of BRCA1. These affect sites involved in formation of the BRCT-n-BRCT-c interface and the binding to phosphoserine-containing peptides. The results suggest that the presence of native BRCT-n and a properly aligned BRCT-n-BRCT-c interface are essential if BRCT-c is to adopt a biologically active conformation. Structural consequences of cancer-associated mutations and biological implications of the dynamic behavior are discussed.

PubMed: 15609993
DOI: 10.1021/bi049550q

実験手法

SOLUTION NMR