1OPO

THE STRUCTURE OF CARNATION MOTTLE VIRUS

1OPO の概要

• エントリーDOI: 10.2210/pdb1opo/pdb
• 関連するPDBエントリー: 2TBV
• 分子名称: Coat protein, SULFATE ION, CALCIUM ION (3 entities in total)
• 機能のキーワード: plant virus, carmovirus, virus/viral protein, tomato bushy stunt virus, icosahedral virus, virus
• 由来する生物種: Carnation mottle virus
• 細胞内の位置: Virion (By similarity): P04383
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 113818.92

構造登録者

Morgunova, E.,Dauter, Z.,Fry, E.,Stuart, D.,Stel'mashchuk, V.,Mikhailov, A.M.,Wilson, K.S.,Vainshtein, B.K. (登録日: 2003-03-06, 公開日: 2003-04-01, 最終更新日: 2023-08-16)

主引用文献

Morgunova, E.,Dauter, Z.,Fry, E.,Stuart, D.,Stel'mashchuk, V.,Mikhailov, A.M.,Wilson, K.S.,Vainshtein, B.K.
The atomic structure of Carnation Mottle Virus capsid protein
Febs Lett., 338:267-271, 1994

PubMed Abstract: The structure of the Carnation Mottle Virus (CMtV) capsid protein has been determined at 3.2 A resolution by the method of molecular replacement. Three-dimensional data were collected from a small number of crystals (sp.g. I23, a = 382.6 A) using the synchrotron radiation with an image plate as detector. The coordinates of Tomato Bushy Stunt Virus (TBSV) were used as a searching model. Refinement of the coordinates of 7,479 non-hydrogen atoms performed by the program XPLOR, has led to an R-factor of 18.3%. It was found that the amino acid chain fold of capsid protein is very similar to that in other icosahedral viruses. However, there are some differences in the contact regions between protein subunits and also the lack of the beta-annulus around the 3-fold icosahedral axes. The structural and biochemical results lead us to consider an alternative assembly pathway.

PubMed: 8307192
DOI: 10.1016/0014-5793(94)80281-5

実験手法

X-RAY DIFFRACTION (3.2 Å)