1OMY

Crystal Structure of a Recombinant alpha-insect Toxin BmKaIT1 from the scorpion Buthus martensii Karsch

1OMY の概要

• エントリーDOI: 10.2210/pdb1omy/pdb
• 分子名称: Alpha-neurotoxin TX12, CHLORIDE ION, ACETIC ACID, ... (4 entities in total)
• 機能のキーワード: alpha-insect toxin, sodium channel, toxin
• 由来する生物種: Mesobuthus martensii (Chinese scorpion)
• 細胞内の位置: Secreted: Q9GQW3
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7425.92

構造登録者

Huang, Y.,Huang, Q.,Chen, H.,Tang, Y.,Miyake, H.,Kusunoki, M. (登録日: 2003-02-26, 公開日: 2003-09-09, 最終更新日: 2024-10-30)

主引用文献

Huang, Y.,Huang, Q.,Chen, H.,Tang, Y.,Miyake, H.,Kusunoki, M.
Crystallization and preliminary crystallographic study of rBmKalphaIT1, a recombinant alpha-insect toxin from the scorpion Buthus martensii Karsch.
Acta Crystallogr.,Sect.D, 59:1635-1636, 2003

PubMed Abstract: Alpha-insect scorpion toxins are a distinct group of scorpion neurotoxins for which no crystal structures are yet available. A novel alpha-insect toxin named BmKalphaIT1 from the scorpion Buthus martensii Karsch (BmK) has been expressed as an inclusion body in Escherichia coli and purified by chromatography after renaturation. Recombinant BmKalphaIT1 (rBmKaIT1) was crystallized using the vapour-diffusion technique in hanging drops at 296 K. The crystals, which were grown in sodium phosphate, belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 30.24 (1), b = 36.51 (3), c = 57.08 (2) A. Diffraction data were collected to 2.1 A resolution using synchrotron radiation. There appears to be one rBmKalphaIT1 molecule in the asymmetric unit.

PubMed: 12925796
DOI: 10.1107/S090744490301415X

実験手法

X-RAY DIFFRACTION (2 Å)