1OLO

Hexameric Replicative DNA Helicase RepA from Plasmid RSF1010 - Cubic Crystal Structure

1OLO の概要

• エントリーDOI: 10.2210/pdb1olo/pdb
• 関連するPDBエントリー: 1G8Y 1NLF
• 分子名称: REGULATORY PROTEIN REPA, SULFATE ION (3 entities in total)
• 機能のキーワード: dna helicase, atpase, motor protein, transcription
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60082.50

構造登録者

Niedenzu, T.,Saenger, W. (登録日: 2003-08-08, 公開日: 2003-09-19, 最終更新日: 2023-12-13)

主引用文献

Ziegelin, G.,Niedenzu, T.,Lurz, R.,Saenger, W.,Lanka, E.
Hexameric Rsf1010 Helicase Repa: The Structural and Functional Importance of Single Amino Acid Residues
Nucleic Acids Res., 31:5917-, 2003

PubMed Abstract: In the known monoclinic crystals the 3-dimensional structure of the hexameric, replicative helicase RepA encoded by plasmid RSF1010 shows 6-fold rotational symmetry. In contrast, in the cubic crystal form at 2.55 A resolution described here RepA has 3-fold symmetry and consists of a trimer of dimers. To study structure-function relationships, a series of repA deletion mutants and mutations yielding single amino acid exchanges were constructed and the respective gene products were analyzed in vivo and in vitro. Hexamerization of RepA occurs via the N-terminus and is required for NTP hydrolysis. The C-terminus is essential both for the interaction with the replication machinery and for the helicase activity. Functional analyses of RepA variants with single amino acid exchanges confirmed most of the predictions that were based on the published 3-dimensional structure. Of the five motifs conserved in family 4 helicases, all residues conserved in RepA and T7 gp4 helicases participate in DNA unwinding. Residues K42, E76, D77, D139 and H178, proposed to play key roles in catalyzing the hydrolysis of NTPs, are essential for RepA activity. Residue H178 of motif H3 couples nucleotide consumption to DNA strand separation.

PubMed: 14530440
DOI: 10.1093/NAR/GKG790

実験手法

X-RAY DIFFRACTION (2.55 Å)