1OJG

Sensory domain of the membraneous two-component fumarate sensor DcuS of E. coli

1OJG の概要

• エントリーDOI: 10.2210/pdb1ojg/pdb
• 分子名称: SENSOR PROTEIN DCUS (1 entity in total)
• 機能のキーワード: transferase, fumarate, dcus, histidine kinase
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15099.13

構造登録者

Pappalardo, L.,Janausch, I.G.,Vijayan, V.,Zientz, E.,Junker, J.,Peti, W.,Zweckstetter, M.,Unden, G.,Griesinger, C. (登録日: 2003-07-10, 公開日: 2003-08-15, 最終更新日: 2024-05-15)

主引用文献

Pappalardo, L.,Janausch, I.G.,Vijayan, V.,Zientz, E.,Junker, J.,Peti, W.,Zweckstetter, M.,Unden, G.,Griesinger, C.
The NMR structure of the sensory domain of the membranous two-component fumarate sensor (histidine protein kinase) DcuS of Escherichia coli.
J. Biol. Chem., 278:39185-39188, 2003

PubMed Abstract: The structure of the water-soluble, periplasmic domain of the fumarate sensor DcuS (DcuS-pd) has been determined by NMR spectroscopy in solution. DcuS is a prototype for a sensory histidine kinase with transmembrane signal transfer. DcuS belongs to the CitA family of sensors that are specific for sensing di- and tricarboxylates. The periplasmic domain is folded autonomously and shows helices at the N and the C terminus, suggesting direct linking or connection to helices in the two transmembrane regions. The structure constitutes a novel fold. The nearest structural neighbor is the Per-Arnt-Sim domain of the photoactive yellow protein that binds small molecules covalently. Residues Arg107, His110, and Arg147 are essential for fumarate sensing and are found clustered together. The structure constitutes the first periplasmic domain of a two component sensory system and is distinctly different from the aspartate sensory domain of the Tar chemotaxis sensor.

PubMed: 12907689
DOI: 10.1074/jbc.C300344200

実験手法

SOLUTION NMR