1OEY

Heterodimer of p40phox and p67phox PB1 domains from human NADPH oxidase

1OEY の概要

• エントリーDOI: 10.2210/pdb1oey/pdb
• 関連するPDBエントリー: 1E96 1H6H 1HH8 1IP9 1IPG 1K4U
• 分子名称: NEUTROPHIL CYTOSOL FACTOR 2, NEUTROPHIL CYTOSOL FACTOR 4 (3 entities in total)
• 機能のキーワード: immune system, pb1 heterodimer-complex, nadph oxidase, pb1 domain, heterodimerization
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Cytoplasm: P19878; Cytoplasm, cytosol : Q15080
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 91155.61

構造登録者

Wilson, M.I.,Gill, D.J.,Perisic, O.,Quinn, M.T.,Williams, R.L. (登録日: 2003-04-02, 公開日: 2003-07-29, 最終更新日: 2024-10-16)

主引用文献

Wilson, M.I.,Gill, D.J.,Perisic, O.,Quinn, M.T.,Williams, R.L.
Pb1 Domain-Mediated Heterodimerization in Nadph Oxidase and Signaling Complexes of Atypical Protein Kinase C with Par6 and P62
Mol.Cell, 12:39-, 2003

PubMed Abstract: Maximal activation of NADPH oxidase requires formation of a complex between the p40(phox) and p67(phox) subunits via association of their PB1 domains. We have determined the crystal structure of the p40(phox)/p67(phox) PB1 heterodimer, which reveals that both domains have a beta grasp topology and that they bind in a front-to-back arrangement through conserved electrostatic interactions between an acidic OPCA motif on p40(phox) and basic residues in p67(phox). The structure enabled us to identify residues critical for heterodimerization among other members of the PB1 domain family, including the atypical protein kinase C zeta (PKC zeta) and its partners Par6 and p62 (ZIP, sequestosome). Both Par6 and p62 use their basic "back" to interact with the OPCA motif on the "front" of the PKC zeta. Besides heterodimeric interactions, some PB1 domains, like the p62 PB1, can make homotypic front-to-back arrays.

PubMed: 12887891
DOI: 10.1016/S1097-2765(03)00246-6

実験手法

X-RAY DIFFRACTION (2 Å)