1ODH

Structure of the GCM domain bound to DNA

1ODH の概要

• エントリーDOI: 10.2210/pdb1odh/pdb
• 分子名称: MGCM1, 5'-D(*CP*GP*AP*TP*GP*CP*GP*GP*GP*TP *GP*CP*A)-3', 5'-D(*TP*GP*CP*AP*CP*CP*CP*GP*CP*AP *TP*CP*G)-3', ... (5 entities in total)
• 機能のキーワード: transcription factor/dna, transcription factor, dna-binding domain, protein-dna complex, transcription factor-dna complex
• 由来する生物種: MUS MUSCULUS (MOUSE)
• 細胞内の位置: Nucleus (Potential): P70348
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 28539.21

構造登録者

Cohen, S.X.,Muller, C.W. (登録日: 2003-02-19, 公開日: 2003-04-08, 最終更新日: 2024-05-08)

主引用文献

Cohen, S.X.,Moulin, M.,Hashemolhosseini, S.,Kilian, K.,Wegner, M.,Muller, C.W.
Crystal Structure of the Gcm Domain-DNA Complex: A DNA-Binding Domain with a Novel Fold and Mode of Target Site Recognition
Embo J., 22:1835-, 2003

PubMed Abstract: Glia cell missing (GCM) transcription factors form a small family of transcriptional regulators in metazoans. The prototypical Drosophila GCM protein directs the differentiation of neuron precursor cells into glia cells, whereas mammalian GCM proteins are involved in placenta and parathyroid development. GCM proteins share a highly conserved 150 amino acid residue region responsible for DNA binding, known as the GCM domain. Here we present the crystal structure of the GCM domain from murine GCMa bound to its octameric DNA target site at 2.85 A resolution. The GCM domain exhibits a novel fold consisting of two domains tethered together by one of two structural Zn ions. We observe the novel use of a beta-sheet in DNA recognition, whereby a five- stranded beta-sheet protrudes into the major groove perpendicular to the DNA axis. The structure combined with mutational analysis of the target site and of DNA-contacting residues provides insight into DNA recognition by this new type of Zn-containing DNA-binding domain.

PubMed: 12682016
DOI: 10.1093/EMBOJ/CDG182

実験手法

X-RAY DIFFRACTION (2.85 Å)