1OCW

Free conformation Ab2 of the IgE SPE-7

1OCW の概要

• エントリーDOI: 10.2210/pdb1ocw/pdb
• 関連するPDBエントリー: 1OAQ 1OAR 1OAU 1OAX 1OAY 1OAZ
• 分子名称: IMMUNOGLOBULIN E (3 entities in total)
• 機能のキーワード: antibody, allergy, conformational diversity, immune system, multispecificity
• 由来する生物種: MUS MUSCULUS (HOUSE MOUSE); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26201.22

構造登録者

James, L.C.,Roversi, P.,Tawfik, D. (登録日: 2003-02-11, 公開日: 2004-02-05, 最終更新日: 2023-12-13)

主引用文献

James, L.C.,Roversi, P.,Tawfik, D.
Antibody Multispecificity Mediated by Conformational Diversity
Science, 299:1362-, 2003

PubMed Abstract: A single antibody was shown to adopt different binding-site conformations and thereby bind unrelated antigens. Analysis by both x-ray crystallography and pre-steady-state kinetics revealed an equilibrium between different preexisting isomers, one of which possessed a promiscuous, low-affinity binding site for aromatic ligands, including the immunizing hapten. A subsequent induced-fit isomerization led to high-affinity complexes with a deep and narrow binding site. A protein antigen identified by repertoire selection made use of an unrelated antibody isomer with a wide, shallow binding site. Conformational diversity, whereby one sequence adopts multiple structures and multiple functions, can increase the effective size of the antibody repertoire but may also lead to autoimmunity and allergy.

PubMed: 12610298
DOI: 10.1126/SCIENCE.1079731

実験手法

X-RAY DIFFRACTION (2 Å)