1OCT

CRYSTAL STRUCTURE OF THE OCT-1 POU DOMAIN BOUND TO AN OCTAMER SITE: DNA RECOGNITION WITH TETHERED DNA-BINDING MODULES

1OCT の概要

• エントリーDOI: 10.2210/pdb1oct/pdb
• 分子名称: DNA (5'-D(*TP*GP*TP*AP*TP*GP*CP*AP*AP*AP*TP*AP*AP*GP*G)-3'), DNA (5'-D(*AP*CP*CP*TP*TP*AP*TP*TP*TP*GP*CP*AP*TP*AP*C)-3'), PROTEIN (OCT-1 POU DOMAIN) (3 entities in total)
• 機能のキーワード: protein-dna complex, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P14859
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 27190.64

構造登録者

Klemm, J.D.,Rould, M.A.,Aurora, R.,Herr, W.,Pabo, C.O. (登録日: 1994-05-09, 公開日: 1994-08-31, 最終更新日: 2024-02-14)

主引用文献

Klemm, J.D.,Rould, M.A.,Aurora, R.,Herr, W.,Pabo, C.O.
Crystal structure of the Oct-1 POU domain bound to an octamer site: DNA recognition with tethered DNA-binding modules.
Cell(Cambridge,Mass.), 77:21-32, 1994

PubMed Abstract: The structure of an Oct-1 POU domain-octamer DNA complex has been solved at 3.0 A resolution. The POU-specific domain contacts the 5' half of this site (ATGCAAAT), and as predicted from nuclear magnetic resonance studies, the structure, docking, and contacts are remarkably similar to those of the lambda and 434 repressors. The POU homeodomain contacts the 3' half of this site (ATGCAAAT), and the docking is similar to that of the engrailed, MAT alpha 2, and Antennapedia homeodomains. The linker region is not visible and there are no protein-protein contacts between the domains, but overlapping phosphate contacts near the center of the octamer site may favor cooperative binding. This novel arrangement raises important questions about cooperativity in protein-DNA recognition.

PubMed: 8156594
DOI: 10.1016/0092-8674(94)90231-3

実験手法

X-RAY DIFFRACTION (3 Å)