1OAN

Crystal structure of the dengue 2 virus envelope protein

1OAN の概要

• エントリーDOI: 10.2210/pdb1oan/pdb
• 関連するPDBエントリー: 1OAM
• 分子名称: ENVELOPE GLYCOPROTEIN, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: glycoprotein, dengue virus, membrane fusion, flavivirus, fusion peptide, low-ph conformational change
• 由来する生物種: DENGUE VIRUS TYPE 2
• 細胞内の位置: Capsid protein C: Virion (Potential). Peptide pr: Secreted (By similarity). Small envelope protein M: Virion membrane; Multi-pass membrane protein (By similarity). Envelope protein E: Virion membrane; Multi- pass membrane protein (By similarity). Non-structural protein 1: Secreted. Non-structural protein 2A-alpha: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Non-structural protein 2A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Non-structural protein 4A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P12823
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 89569.55

構造登録者

Modis, Y.,Harrison, S.C. (登録日: 2003-01-16, 公開日: 2003-05-16, 最終更新日: 2024-11-13)

主引用文献

Modis, Y.,Ogata, S.,Clements, D.,Harrison, S.C.
A Ligand-Binding Pocket in the Dengue Virus Envelope Glycoprotein
Proc.Natl.Acad.Sci.USA, 100:6986-, 2003

PubMed Abstract: Dengue virus is an emerging global health threat. Its major envelope glycoprotein, E, mediates viral attachment and entry by membrane fusion. A crystal structure of the soluble ectodomain of E from dengue virus type 2 reveals a hydrophobic pocket lined by residues that influence the pH threshold for fusion. The pocket, which accepts a hydrophobic ligand, opens and closes through a conformational shift in a beta-hairpin at the interface between two domains. These features point to a structural pathway for the fusion-activating transition and suggest a strategy for finding small-molecule inhibitors of dengue and other flaviviruses.

PubMed: 12759475
DOI: 10.1073/PNAS.0832193100

実験手法

X-RAY DIFFRACTION (2.75 Å)