1O9G

rRNA methyltransferase aviRa from Streptomyces viridochromogenes at 1.5A

1O9G の概要

• エントリーDOI: 10.2210/pdb1o9g/pdb
• 関連するPDBエントリー: 1O9G 1QAM 1QAN 1QAO 1QAQ 1YUB
• 分子名称: RRNA METHYLTRANSFERASE (2 entities in total)
• 機能のキーワード: transferase, antibiotic resistance; rrna-methyltransferase, se-mad
• 由来する生物種: STREPTOMYCES VIRIDOCHROMOGENES
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26833.49

構造登録者

Mosbacher, T.G.,Schulz, G.E. (登録日: 2002-12-13, 公開日: 2003-05-16, 最終更新日: 2024-10-23)

主引用文献

Mosbacher, T.G.,Bechthold, A.,Schulz, G.E.
Crystal Structure of the Avilamycin Resistance-Conferring Methyltransferase Avira from Streptomyces Viridochromogenes
J.Mol.Biol., 329:147-, 2003

PubMed Abstract: The emergence of antibiotic-resistant bacterial strains is a widespread problem in contemporary medical practice and drug design. It is therefore important to elucidate the underlying mechanism in each case. The methyltransferase AviRa from Streptomyces viridochromogenes mediates resistance to the antibiotic avilamycin, which is closely related to evernimicin, an oligosaccharide antibiotic that has been used in medical studies. The structure of AviRa was determined by X-ray diffraction at 1.5A resolution. Phases were obtained from one selenomethionine residue introduced by site-directed mutagenesis. The chain-fold is similar to that of most methyltransferases, although AviRa contains two additional helices as a specific feature. A putative-binding site for the cofactor S-adenosyl-L-methionine was derived from homologous structures. It agrees with the conserved pattern of interacting amino acid residues. AviRa methylates a specific guanine base within the peptidyltransferase loop of the 23S ribosomal RNA. Guided by the target, the enzyme was docked to the cognate ribosomal surface, where it fit well into a deep cleft without contacting any ribosomal protein. The two additional alpha-helices of AviRa filled a depression in the surface. Since the transferred methyl group of the cofactor is in a pocket beneath the enzyme surface, the targeted guanine base has to flip out for methylation.

PubMed: 12742024
DOI: 10.1016/S0022-2836(03)00407-8

実験手法

X-RAY DIFFRACTION (1.5 Å)