1O7L

Molybdate-activated form of ModE from Escherichia coli

1O7L の概要

• エントリーDOI: 10.2210/pdb1o7l/pdb
• 関連するPDBエントリー: 1B9M 1B9N 1H9R 1H9S
• 分子名称: TRANSCRIPTIONAL REGULATOR MODE, MOLYBDATE ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: transcription regulation, dna binding, molybdate, mop, winged helix-turn-helix, transcriptional regulator, activator
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Cytoplasm: P46930
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 114107.80

構造登録者

Schuttelkopf, A.W.,Hunter, W.N. (登録日: 2002-11-08, 公開日: 2003-02-20, 最終更新日: 2023-12-13)

主引用文献

Schuttelkopf, A.W.,Boxer, D.H.,Hunter, W.N.
Crystal Structure of Activated Mode Reveals Conformational Changes Involving Both Oxyanion and DNA-Binding Domains
J.Mol.Biol., 326:761-, 2003

PubMed Abstract: ModE is a bacterial transcriptional regulator that orchestrates many aspects of molybdenum metabolism by binding to specific DNA sequences in a molybdate-dependent fashion. We present the crystal structure of Escherichia coli ModE in complex with molybdate, which was determined at 2.75A from a merohedrally twinned crystal (twin fraction approximately 0.30) with space group P4(3). We now have structures of ModE in both its "switched on" (ligand-bound) and "switched off" (apo) states. Comparison with the apo structure shows that ligand binding leads to extensive conformational changes not only in the molybdate-binding domain, but also in the DNA-binding domain. The most obvious difference is the loss of the pronounced asymmetry between the two chains of the ModE dimer, which had been a characteristic property of the apo structure. Another major change concerns the relative orientation of the two DNA-interacting winged helix-turn-helix motifs. Manual docking of an idealized DNA structure suggests that this conformational change should improve DNA binding of the activated molybdate-bound ModE.

PubMed: 12581638
DOI: 10.1016/S0022-2836(02)01358-X

実験手法

X-RAY DIFFRACTION (2.75 Å)