1NX2

Calpain Domain VI

1NX2 の概要

• エントリーDOI: 10.2210/pdb1nx2/pdb
• 関連するPDBエントリー: 1NX0 1NX1 1NX3
• 分子名称: Calcium-dependent protease, small subunit, CALCIUM ION (3 entities in total)
• 機能のキーワード: hydrolase, calcium binding
• 由来する生物種: Sus scrofa (pig)
• 細胞内の位置: Cytoplasm (By similarity): P04574
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20043.79

構造登録者

Todd, B.,Moore, D.,Deivanayagam, C.C.S.,Lin, G.-D.,Chattopadhyay, D.,Maki, M.,Wang, K.K.W.,Narayana, S.V.L. (登録日: 2003-02-07, 公開日: 2003-08-19, 最終更新日: 2024-02-14)

主引用文献

Todd, B.,Moore, D.,Deivanayagam, C.C.S.,Lin, G.-D.,Chattopadhyay, D.,Maki, M.,Wang, K.K.W.,Narayana, S.V.L.
A structural model for the inhibition of calpain by calpastatin: crystal structures of the native domain VI of calpain and its complexes with calpastatin peptide and a small molecule inhibitor.
J.Mol.Biol., 328:131-146, 2003

PubMed Abstract: The Ca(2+)-dependent cysteine protease calpain along with its endogenous inhibitor calpastatin is widely distributed. The interactions between calpain and calpastatin have been studied to better understand the nature of calpain inhibition by calpastatin, which can aid the design of small molecule inhibitors to calpain. Here we present the crystal structure of a complex between a calpastatin peptide and the calcium-binding domain VI of calpain. DIC19 is a 19 residue peptide, which corresponds to one of the three interacting domains of calpastatin, which is known to interact with domain VI of calpain. We present two crystal structures of DIC19 bound to domain VI of calpain, determined by molecular replacement methods to 2.5A and 2.2A resolution. In the process of crystallizing the inhibitor complex, a new native crystal form was identified which had the homodimer 2-fold axis along a crystallographic axis as opposed to the previously observed dimer in the asymmetric unit. The crystal structures of the native domain VI and its inhibitor PD150606 (3-(4-iodophenyl)-2-mercapto-(Z)-2-propenoic acid) complex were determined with the help of molecular replacement methods to 2.0A and 2.3A resolution, respectively. In addition, we built a homology model for the complex between domain IV and DIA19 peptide of calpastatin. Finally, we present a model for the calpastatin-inhibited calpain.

PubMed: 12684003
DOI: 10.1016/S0022-2836(03)00274-2

実験手法

X-RAY DIFFRACTION (2.2 Å)