1NUG

Role of Calcium Ions in the Activation and Activity of the Transglutaminase 3 Enzyme (2 calciums, 1 Mg, inactive form)

1NUG の概要

• エントリーDOI: 10.2210/pdb1nug/pdb
• 関連するPDBエントリー: 1L9M 1L9N 1NUD 1NUF
• 分子名称: Protein-glutamine glutamyltransferase E, CHLORIDE ION, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: transglutaminase 3, metalloenzyme, calcium ion, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 153691.73

構造登録者

Ahvazi, B. (登録日: 2003-01-31, 公開日: 2003-04-22, 最終更新日: 2023-08-16)

主引用文献

Ahvazi, B.,Boeshans, K.M.,Idler, W.,Baxa, U.,Steinert, P.M.
Roles of Calcium Ions in the Activation and Activity of the Transglutaminase 3 Enzyme
J.Biol.Chem., 278:23834-23841, 2003

PubMed Abstract: The transglutaminase 3 enzyme is widely expressed in many tissues including epithelia. We have shown previously that it can bind three Ca2+ ions, which in site one is constitutively bound, while those in sites two and three are acquired during activation and are required for activity. In particular, binding at site three opens a channel through the enzyme and exposes two tryptophan residues near the active site that are thought to be important for enzyme reaction. In this study, we have solved the structures of three more forms of this enzyme by x-ray crystallography in the presence of Ca2+ and/or Mg2+, which provide new insights on the precise contribution of each Ca2+ ion to activation and activity. First, we found that Ca2+ ion in site one can be exchanged with difficulty, and it has a binding affinity of Kd = 0.3 microm (DeltaH = -6.70 +/- 0.52 kcal/mol), which suggests it is important for the stabilization of the enzyme. Site two can be occupied by some lanthanides but only Ca2+ of the Group 2 family of alkali earth metals, and its occupancy are required for activity. Site three can be occupied by some lanthanides, Ca2+,or Mg2+; however, when Mg2+ is present, the enzyme is inactive, and the channel is closed. Thus Ca2+ binding in both sites two and three cooperate in opening the channel. We speculate that manipulation of the channel opening could be controlled by intracellular cation levels. Together, these data have important implications for reaction mechanism of the enzyme: the opening of a channel perhaps controls access to and manipulation of substrates at the active site.

PubMed: 12679341
DOI: 10.1074/jbc.M301162200

実験手法

X-RAY DIFFRACTION (2.4 Å)