1NTX

SECONDARY STRUCTURE DETERMINATION FOR ALPHA-NEUROTOXIN FROM DENDROASPIS POLYLEPIS POLYLEPIS BASED ON SEQUENCE SPECIFIC PROTON NUCLEAR MAGNETIC RESONANCE ASSIGNMENTS

1NTX の概要

• エントリーDOI: 10.2210/pdb1ntx/pdb
• 分子名称: ALPHA-NEUROTOXIN (1 entity in total)
• 機能のキーワード: neurotoxin
• 由来する生物種: Dendroaspis polylepis polylepis (black mamba)
• 細胞内の位置: Secreted: P01416
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6928.92

構造登録者

Brown, L.R.,Wuthrich, K. (登録日: 1992-04-30, 公開日: 1994-01-31, 最終更新日: 2024-10-30)

主引用文献

Labhardt, A.M.,Hunziker-Kwik, E.H.,Wuthrich, K.
Secondary structure determination for alpha-neurotoxin from Dendroaspis polylepis polylepis based on sequence-specific 1H-nuclear-magnetic-resonance assignments.
Eur.J.Biochem., 177:295-305, 1988

PubMed Abstract: Sequence-specific assignments are presented for the polypeptide backbone protons and a majority of the amino-acid-side-chain protons of alpha-neurotoxin from Dendroaspis polylepis polylepis, and individual amide proton-exchange rates with the solvent are reported. The secondary structure and the hydrogen-bonding patterns in the regular secondary structure elements are deduced from nuclear Overhauser effects and the sequence locations of the slowly exchanging amide protons. The molecule includes a three-stranded antiparallel beta-sheet, and there are indications that two additional short chain segments are arranged in an antiparallel beta-sheet. These structural elements are similar, but not identical, to either the secondary structure reported for erabutoxin b in single crystals, or the solution structure of cytotoxin CTXIIb from Naja mossambica mossambica.

PubMed: 2847926
DOI: 10.1111/j.1432-1033.1988.tb14376.x

実験手法

SOLUTION NMR