1NTH

Crystal structure of the methanosarcina barkeri monomethylamine methyltransferase (MTMB)

Replaces:  1L2R

Summary for 1NTH

• Entry DOI: 10.2210/pdb1nth/pdb
• Related: 1L2Q
• Descriptor: Monomethylamine methyltransferase mtmB1 (2 entities in total)
• Functional Keywords: tim barrel, transferase
• Biological source: Methanosarcina barkeri
• Total number of polymer chains: 1
• Total formula weight: 50291.23

Authors

Hao, B.,Gong, W.,Ferguson, T.K.,James, C.M.,Krzycki, J.A.,Chan, M.K. (deposition date: 2003-01-30, release date: 2003-02-04, Last modification date: 2024-02-14)

Primary citation

Hao, B.,GONG, W.,FERGUSON, T.K.,JAMES, C.M.,KRZYCKI, J.A.,Chan, M.K.
A new UAG-encoded residue in the structure of a methanogen methyltransferase
Science, 296:1462-1466, 2002

PubMed Abstract: Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine.

PubMed: 12029132
DOI: 10.1126/science.1069556

Experimental method

X-RAY DIFFRACTION (1.55 Å)