1NQP

Crystal structure of Human hemoglobin E at 1.73 A resolution

1NQP の概要

• エントリーDOI: 10.2210/pdb1nqp/pdb
• 関連するPDBエントリー: 1BBB
• 分子名称: Hemoglobin alpha chain, Hemoglobin beta chain, CYANIDE ION, ... (5 entities in total)
• 機能のキーワード: hemoglobin e oxygen transport beta thalassemia, oxygen storage-transport complex, oxygen storage/transport
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64651.25

構造登録者

Dasgupta, J.,Sen, U.,Choudhury, D.,Dutta, P.,Basu, S.,Chakrabarti, A.,Chakrabarty, A.,Dattagupta, J.K. (登録日: 2003-01-22, 公開日: 2004-03-02, 最終更新日: 2023-08-16)

主引用文献

Dasgupta, J.,Sen, U.,Choudhury, D.,Dutta, P.,Chakrabarti, A.,Chakrabarty, S.B.,Chakrabarty, A.,Dattagupta, J.K.
Crystallization and preliminary X-ray structural Studies of Hemoglobin A2 and Hemoglobin E, isolated from the blood samples of Beta-thalassemic patients
Biochem.Biophys.Res.Commun., 303:619-623, 2004

PubMed Abstract: Hemoglobin A(2) (alpha(2)delta(2)), a minor (2-3%) component of circulating red blood cells, acts as an anti-sickling agent and its elevated concentration in beta-thalassemia is a useful clinical diagnostic. In beta-thalassemia major, where there is a failure of beta-chain production, HbA(2) acts as the predominant oxygen delivery mechanism. Hemoglobin E, is another common abnormal hemoglobin, caused by splice site mutation in exon 1 of beta globin gene, when combines with beta-thalassemia, causes severe microcytic anemia. The purification, crystallization, and preliminary structural studies of HbA(2) and HbE are reported here. HbA(2) and HbE are purified by cation exchange column chromatography in presence of KCN from the blood samples of individuals suffering from beta-thalassemia minor and E beta-thalassemia. X-ray diffraction data of HbA(2) and HbE were collected upto 2.1 and 1.73 A, respectively. HbA(2) crystallized in space group P2(1) with unit cell parameters a=54.33 A, b=83.73 A, c=62.87 A, and beta=99.80 degrees whereas HbE crystallized in space group P2(1)2(1)2(1) with unit cell parameters a=60.89 A, b=95.81 A, and c=99.08 A. Asymmetric unit in each case contains one Hb tetramer in R(2) state.

PubMed: 12659864
DOI: 10.1016/S0006-291X(03)00379-6

実験手法

X-RAY DIFFRACTION (1.73 Å)