1NPT

Glyceraldehyde-3-Phosphate Dehydrogenase Mutant With Cys 149 replaced by Ala complexed with NAD+

1NPT の概要

• エントリーDOI: 10.2210/pdb1npt/pdb
• 関連するPDBエントリー: 1NQ5 1NQA 1NQO
• 分子名称: Glyceraldehyde 3-phosphate dehydrogenase, SULFATE ION, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (4 entities in total)
• 機能のキーワード: glycolysis, oxidoreductase, nad
• 由来する生物種: Geobacillus stearothermophilus
• 細胞内の位置: Cytoplasm: P00362
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 146873.89

構造登録者

Didierjean, C.,Corbier, C.,Fatih, M.,Favier, F.,Boschi-Muller, S.,Branlant, G.,Aubry, A. (登録日: 2003-01-20, 公開日: 2003-04-22, 最終更新日: 2023-08-16)

主引用文献

Didierjean, C.,Corbier, C.,Fatih, M.,Favier, F.,Boschi-Muller, S.,Branlant, G.,Aubry, A.
Crystal structure of two ternary complexes of phosphorylating Glyceraldehyde-3-Phosphate Dehydrogenase from Bacillus stearothermophilus with NAD and D-Glyceraldehyde-3-Phosphate
J.Biol.Chem., 278:12968-12976, 2003

PubMed Abstract: The crystal structure of the phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Bacillus stearothermophilus was solved in complex with its cofactor, NAD, and its physiological substrate, D-glyceraldehyde 3-phosphate (D-G3P). To isolate a stable ternary complex, the nucleophilic residue of the active site, Cys(149), was substituted with alanine or serine. The C149A and C149S GAPDH ternary complexes were obtained by soaking the crystals of the corresponding binary complexes (enzyme.NAD) in a solution containing G3P. The structures of the two binary and the two ternary complexes are presented. The D-G3P adopts the same conformation in the two ternary complexes. It is bound in a non-covalent way, in the free aldehyde form, its C-3 phosphate group being positioned in the P(s) site and not in the P(i) site. Its C-1 carbonyl oxygen points toward the essential His(176), which supports the role proposed for this residue along the two steps of the catalytic pathway. Arguments are provided that the structures reported here are representative of a productive enzyme.NAD.D-G3P complex in the ground state (Michaelis complex).

PubMed: 12569100
DOI: 10.1074/jbc.M211040200

実験手法

X-RAY DIFFRACTION (2.18 Å)