1NPO

BOVINE NEUROPHYSIN II COMPLEX WITH OXYTOCIN

1NPO の概要

• エントリーDOI: 10.2210/pdb1npo/pdb
• 分子名称: NEUROPHYSIN II, OXYTOCIN (3 entities in total)
• 機能のキーワード: complex (hormone transport-hormone), hypothalamus, complex (hormone transport-hormone) complex, complex (hormone transport/hormone)
• 由来する生物種: Bos taurus (cattle); 詳細
• 細胞内の位置: Secreted: P01180 P01180
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 21742.78

構造登録者

Rose, J.P.,Wang, B.-C. (登録日: 1996-02-01, 公開日: 1997-02-12, 最終更新日: 2024-10-30)

主引用文献

Rose, J.P.,Wu, C.K.,Hsiao, C.D.,Breslow, E.,Wang, B.C.
Crystal structure of the neurophysin-oxytocin complex.
Nat.Struct.Biol., 3:163-169, 1996

PubMed Abstract: The first crystal structure of the pituitary hormone oxytocin complexed with its carrier protein neurophysin has been determined and refined to 3.0 A resolution. The hormone-binding site is located at the end of a 3(10)-helix and involves residues from both domains of each monomer. Hormone residues Tyr 2, which is buried deep in the binding pocket, and Cys 1 have been confirmed as the key residues involved in neurophysin-hormone recognition. We have compared the bound oxytocin observed in the neurophysin-oxytocin complex, the X-ray structures of unbound oxytocin analogues and the NMR-derived structure for bound oxytocin. We find that while our structure is in agreement with the previous crystallographic findings, it differs from the NMR result with regard to how Tyr 2 of the hormone is recognized by neurophysin.

PubMed: 8564543
DOI: 10.1038/nsb0296-163

実験手法

X-RAY DIFFRACTION (3 Å)