1NH2

Crystal structure of a yeast TFIIA/TBP/DNA complex

1NH2 の概要

• エントリーDOI: 10.2210/pdb1nh2/pdb
• 分子名称: 5'-D(*TP*GP*TP*AP*(5IU)P*GP*TP*AP*TP*AP*(5IU)P*AP*AP*AP*AP*C)-3', 5'-D(*GP*TP*TP*TP*TP*AP*TP*AP*TP*AP*CP*AP*TP*AP*CP*A)-3', Transcription initiation factor TFIID, ... (7 entities in total)
• 機能のキーワード: transcription/dna, transcription-dna complex
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Nucleus: P13393; Cytoplasm: P32773 P32774 P32773
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 58529.21

構造登録者

Bleichenbacher, M.,Tan, S.,Richmond, T.J. (登録日: 2002-12-18, 公開日: 2003-10-21, 最終更新日: 2024-05-22)

主引用文献

Bleichenbacher, M.,Tan, S.,Richmond, T.J.
Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes.
J.Mol.Biol., 332:783-793, 2003

PubMed Abstract: RNA polymerase II-dependent transcription requires the assembly of a multi-protein, preinitiation complex on core promoter elements. Transcription factor IID (TFIID) comprising the TATA box-binding protein (TBP) and TBP-associated factors (TAFs) is responsible for promoter recognition in this complex. Subsequent association of TFIIA and TFIIB provides enhanced complex stability. TFIIA is required for transcriptional stimulation by certain viral and cellular activators, and favors formation of the preinitiation complex in the presence of repressor NC2. The X-ray structures of human and yeast TBP/TFIIA/DNA complexes at 2.1A and 1.9A resolution, respectively, are presented here and seen to resemble each other closely. The interactions made by human TFIIA with TBP and DNA within and upstream of the TATA box, including those involving water molecules, are described and compared to the yeast structure. Of particular interest is a previously unobserved region of TFIIA that extends the binding interface with TBP in the yeast, but not in the human complex, and that further elucidates biochemical and genetic results.

PubMed: 12972251
DOI: 10.1016/S0022-2836(03)00887-8

実験手法

X-RAY DIFFRACTION (1.9 Å)